Improved Red-emitting Firefly Luciferase Mutant for Biotechnical Applications
نویسندگان
چکیده
p 3 Introduction pgs 5-10 Materials and Methods pgs 10-18 Materials General Methods Site-directed Mutagenesis Insertion of Promega’s CBRluc into the pGEX-6P-2 vector Protein Expression and Purification Bioluminescence activity-based light assays Heat inactivation studies Bioluminescence emission spectra Microplate luminometer assays Mammalian cell experiments by Promega collaborators: -Transfection of mammalian cells with Luc mutants -Preparation of soluble cell lysates from HEK293 cells expressed at 37 °C -Bioluminescence in mammalian cells Results and Discussion pgs 19-32 Bioluminescence color of luciferase mutants Specific activities of luciferase mutants Heat inactivation studies Microplate luminometer studies Codon-optimization for mammalian cells References pgs 32-34
منابع مشابه
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